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Imaging flow cytometry (IFC) combines the high-throughput capabilities of conventional flow cytometry with single-cell imaging. CellProfiler can be used to analyze the resulting images from imaging flow cytometry, whether brightfield, darkfield, or fluorescence. We here provide an open-source IFC...

A component of CellProfiler cell image analysis software The WormToolbox is a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and...

CellProfiler enables reproducible research because a saved pipeline includes all the modules and settings. Here, we provide links to pipelines used in some published studies, as well as the version of CellProfiler used to create them. You can instead download the most current version of CellProfiler...

OLD CellProfiler example images and pipelines (Prior to 4.0) These examples are outdated, please see our current Examples page for the updated versions. Basic Pipelines Human cells Fruit fly cells Tumors Comet assay Specialized Pipelines Cell/particle counting and scoring the percentage of stained...

CellProfiler Video Tutorials Visit our YouTube playlist for video tutorials on CellProfiler, CellProfiler Analyst, segmentation strategies, how to construct pipelines, and much more. CellProfiler Written Tutorials Technical descriptions of CellProfiler and CellProfiler Analyst software can be found...

Note: CellProfiler versions 2.2 and 3.0 require installation of a Java 8 JDK CellProfiler 4 4.2.7 macOS (10.15+) Windows (64-bit) 4.2.6 macOS (10.15+) Windows (64-bit) 4.2.5 macOS (10.15+) Windows (64-bit) 4.2.4 macOS (10.15+) Windows (64-bit) 4.2.1 macOS (10.14+) Windows (64-bit) 4.1.3 macOS (10.14...

Update September 2024: CellProfiler 4.2.8. release New release for MacOS (Intel and ARM) is available here New release for Windows 10 and 11 is available here. After June 28th, 2023, if you try to download CellProfiler from a very old browser, you might not be able to due to updated AWS security...

CellProfiler Analyst (CPA) provides tools for classifying biological images and exploring and visualizing multidimensional data (particularly from high-throughput experiments) that has been extracted from companion image analysis software CellProfiler. CellProfiler Analyst’s primary tools: Image...

The app requires JDK 1.8 to be installed Stable (2.2.0): January 21, 2016 OS X Windows 32-bit 32-bit Source Nightly (2.2.1): OS X Windows 32-bit 32-bit Source Legacy (2.0.0): OS X Windows 32-bit 32-bit

How to cite CellProfiler in publications (the project's survival depends on it!) CellProfiler for cells (feel free to cite the most recent) : Stirling DR, Swain-Bowden MJ, Lucas AM, Carpenter AE, Cimini BA, Goodman A (2021). CellProfiler 4: improvements in speed, utility and usability. BMC...