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Rat astrocyte cells

Accession number BBBC042 · Version 1 Example images Description of the biological application The morphology of astrocytes is highly heterogeneous and counting them is currently a laborious task. Development of fully automated counting methods for astrocytes from immunohistological images without...

Mouse hippocampal presynaptic terminals (vGlut-1 and vGat)

Accession number BBBC044 · Version 1 Example images Description of the biological application These images are of mouse hippocampal presynaptic terminals and the goal is to extract presynaptic terminals' densities. The presented seet of images were being used to validate the accuracy of automatic...

Human White Blood Cells

Accession number BBBC045 · Version 1 Example images Description of the biological application This dataset is useful for measuring differential White Blood Cell Count. White blood cells play an important role in the immune system to protect against infections and diseases. There are 5 different...

Human HT29 colon-cancer cells shRNAi screen

Accession number BBBC017 · Version 1 Example image Biological application These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. Moffat et al. (Cell, 2006) used the HT29 cell line to screen 4,903 shRNAi...

Human kidney cortex cells

Accession number BBBC051 · Version 1 Example images Biological application The dataset was designed to train a classification algorithm to correctly identify cell types in the human kidney cortex. Images Confocal single channel images of DAPI fluorescence cropped from tissue sections. Images...

Benchmarking Methodology

To reasonably compare, or benchmark, algorithms for image analysis, researchers must agree on a common reference point—the image set's "correct" answer—and see how closely each algorithm matches it. We adopt the common term ground truth for this reference point even though it may be questionable in...

Drosophila Kc167 cells

Accession number BBBC002 · Version 1 Example image Biological application Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and...

Human U2OS cells (out of focus)

Accession number BBBC006 · Version 1 Example images Ground truth: in focus Out of focus Ground truth: foreground Biological application Since robust foreground/background separation and segmentation of cellular objects (i.e., identification of which pixels below to which objects) strongly depends on...

Collective cell migration

Accession number BBBC019 · Version 2 Example images DIC image Ground truth DIC image Ground truth Biological application The wound-healing assay is considered the gold standard for studying cell motility and migration. The assay is performed by time-lapse imaging (usually by phase contrast, or...

3D HL60 Cell line (synthetic data)

Accession number BBBC024 · Version 1 Example images 3D image 3D foreground Biological application One of the principal challenges in counting or segmenting nuclei is dealing with clustered nuclei. To help assess algorithms' performance in this regard, this synthetic image set consists of four...