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Lab Policy and Culture
Photo credit: Juliana Sohn Our mission: To uncover biological knowledge and new medicines by developing advanced methods to quantify and mine the rich information present in images. Our motto: Measure everything, ask questions later. Our ethos: - We love to discover the inner workings of biological...
Training program
The Postdoctoral Training Program in Bioimage Analysis is an opportunity for biologists with strong interest and some experience in image analysis to develop deeper skills through many different individual projects as well as by supporting thousands of researchers around the world who are...
Team
Human MCF7 cells – compound-profiling experiment
Accession number BBBC021 · Version 1 Example Images Actin disrupter Aurora kinase inhbitor Eg5 inhibitor Tubulin destabilizer Tubulin stabilizer Description of the biological application Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each...
Human U2OS cells – Gene overexpression profiling experiment
Accession number BBBC037 · Version 1 Example images Description of the biological application The image set can be used to functionally annotate the genes through comparing their overexpression morphological profiles. Images Images from five fluorescent channels were captured at 20x magnification on...
Imaging flow cytometry analysis using CellProfiler
Imaging flow cytometry (IFC) combines the high-throughput capabilities of conventional flow cytometry with single-cell imaging. CellProfiler can be used to analyze the resulting images from imaging flow cytometry, whether brightfield, darkfield, or fluorescence. We here provide an open-source IFC...
WormToolbox
A component of CellProfiler cell image analysis software The WormToolbox is a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and...
Published pipelines
CellProfiler enables reproducible research because a saved pipeline includes all the modules and settings. Here, we provide links to pipelines used in some published studies, as well as the version of CellProfiler used to create them. You can instead download the most current version of CellProfiler...
Previous Examples
OLD CellProfiler example images and pipelines (Prior to 4.0) These examples are outdated, please see our current Examples page for the updated versions. Basic Pipelines Human cells Fruit fly cells Tumors Comet assay Specialized Pipelines Cell/particle counting and scoring the percentage of stained...