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Join our lab

Any open positions in our lab are always posted on the Broad Institute Careers website and the "Job Opportunities" section of the image.sc forum. When timelines allow, we also share postings on the MicroscopyDB jobs board. We are proud to foster a respectful, multicultural, and interdisciplinary...

Roles within the lab

Postdoctoral Fellows While the majority of our postdocs are members of the training program, we do also train traditional postdoctoral researchers who have one or two specific research projects. We welcome applications from fellows with independent funding at any time; other projects will be...

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CellProfiler Analyst Stable (3.0.5): macOS Windows Source code available on Github Previous CellProfiler Analyst releases

Our Team

The Sheng lab is committed to diversity, equity and inclusion and our lab is dedicated to ensuring a welcoming and safe space for all individuals. We actively work to increase the breadth of our applicant pools to create an environment that supports and retains a richly diverse community...

Tutorials

CellProfiler Video Tutorials Visit our YouTube playlist for video tutorials on CellProfiler, CellProfiler Analyst, segmentation strategies, how to construct pipelines, and much more. CellProfiler Written Tutorials Technical descriptions of CellProfiler and CellProfiler Analyst software can be found...

Mouse Embryos

Accession number BBBC003 · Version 1 Example images . DIC Image Foreground Biological application Fluorescence microscopy cannot be used to image human embryos to determine embryo viability for in vitro fertilization because the introduction of exogenous fluorescent dyes is considered a toxic...

C. elegans live/dead assay

Accession number BBBC010 · Version 1 Example images Positive: mostly alive Negative: mostly dead Biological application This selection of images are controls selected from a screen to find novel anti-infectives using the roundworm C.elegans . The animals were exposed to the pathogen Enterococcus...

Human HT29 colon-cancer cells (diverse phenotypes)

Accession number BBBC018 · Version 1 Example Images ​ ​ ​ ​ ​ Actin blebs Actin dots Anaphase/Telephase Angular Cell Edges Cresent Nuclei ​ ​ ​ ​ ​ Long Projections Metaphase Motile Peas in a Pod Peripheral Actin ​ ​ ​ ​ Large Spread Phospho-histone H3 dots Prometaphase Prophase Biological...

Chinese Hamster Ovary Cells

Accession number BBBC030 · Version 1 Example images DIC image Ground truth Images The image set consists of 60 Differential Interference Contrast (DIC) images of Chinese Hamster Ovary (CHO) cells. The images are taken on an Olympus Cell-R microscope with a 20x lens at the time when the cell...

C. elegans metabolism assay

Accession number BBBC011 · Version 1 Example images Negative control: wild type Positive control: fat Biological application Images are controls from an RNA interference screen for genes affecting fat mass in the nematode C.elegans. The animals are stained with oil red O, a non-flourescentdye which...