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Human U2OS cells cytoplasm–nucleus translocation

Accession number BBBC013 · Version 1 Example images Positive Negative Biological application This 96-well plate has images of cytoplasm to nucleus translocation of the Forkhead (FKHR-EGFP) fusion protein in stably transfected human osteosarcoma cells, U2OS. In proliferating cells, FKHR is localized...

Human U2OS cells transfluor

Accession number BBBC015 · Version 1 Example images Positive Negative Biological application The images are of a human osteosarcoma cell line (U2OS) co-expressing beta2 (b2AR) adrenergic receptor and arrestin-GFP protein molecules. The receptor was modified-type that generates "vesicle-type" spots...

Human U2OS cells transfluor

Accession number BBBC016 · Version 1 Example images Positive Negative Description of the biological application This image set is of a Transfluor assay where an orphan GPCR is stably integrated into the b-arrestin GFP expressing U2OS cell line. After one hour incubation with a compound the cells...

Human MCF7 cells – compound-profiling experiment

Accession number BBBC021 · Version 1 Example Images Actin disrupter Aurora kinase inhbitor Eg5 inhibitor Tubulin destabilizer Tubulin stabilizer Description of the biological application Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each...

Human U2OS cells – Gene overexpression profiling experiment

Accession number BBBC037 · Version 1 Example images Description of the biological application The image set can be used to functionally annotate the genes through comparing their overexpression morphological profiles. Images Images from five fluorescent channels were captured at 20x magnification on...

Published pipelines

CellProfiler enables reproducible research because a saved pipeline includes all the modules and settings. Here, we provide links to pipelines used in some published studies, as well as the version of CellProfiler used to create them. You can instead download the most current version of CellProfiler...

Previous Examples

OLD CellProfiler example images and pipelines (Prior to 4.0) These examples are outdated, please see our current Examples page for the updated versions. Basic Pipelines Human cells Fruit fly cells Tumors Comet assay Specialized Pipelines Cell/particle counting and scoring the percentage of stained...

Resources

The Pan Lab has created PatchAnalytics, a cloud-based web application for automated analysis of SyncroPatch Data. PatchAnalytics Contributors: Lei A. Wang, Ziyue Zhu, David Zhou, David Baez, Yining Wang The Pan Lab has created IntelliSleepScorer, a software package with a graphic user interface for...

Anna Greka MD, PHD

Photo credit: Martin Adolfsson Associate Professor Harvard Medical School Brigham and Women’s Hospital Institute Member Director Kidney Disease Initiative Broad Institute of MIT and Harvard With a focus on laying the foundation for molecularly targeted therapies, Anna’s scientific work is centered...

Merkin Prize Jury

The Merkin Prize Jury is composed of scientific leaders from academia and industry in the US and Europe.