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Help! Why do my output images seem all black?

Blog: March 15, 2017

Beth Cimini Double clicking on the output images produced by CellProfiler sometimes opens up a screen in your operating system’s default image viewer that looks all black. This can make it seem like your pipeline didn’t work or didn’t produce the right output. However, this can happen for a couple...

Meet Our Team

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CellProfiler 4.0 Release: Improvements in speed, utility, and usability

Blog: September 09, 2020

David R. Stirling We have now released CellProfiler version 4.0! Download it here. The primary goal of this release has been to migrate CellProfiler from Python 2 to Python 3. This has involved extensive restructuring of the code base, leading to a lot of “under-the-hood” improvements. Important...

Alán Fernando Muñoz González

Person: Postdoctoral Associate

My interests lie in the interface of computer science, math, image analysis, and biology. I aim to develop tools and workflows to process high throughput imaging datasets, with special interest on applying computational methods on live microscopy data. I am also keen on extracting biological...

Contact

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Email imagingadmin@broadinstitute.org Mailing address Broad Institute of MIT and Harvard415 Main StreetCambridge, MA 02142Our offices are on the 5th floor of 415 Main St [ Google Maps link]. Look for the entrance of our building to the right of the Broad Institute sign.

NSF CAREER project page

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NSF CAREER Award Project (March 2012–February 2017) [ Project abstract at nsf.gov] [Project Outcomes Report (search here, using Fed Award ID 1148823 ( PDF))] Non-technical description of the problem: Biologists want to test genetic and chemical treatments in environments that more closely mimic the...

Anne E. Carpenter

Person: Principal Investigator, Institute Scientist, Senior Director of the Imaging Platform

Group Alumni

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Where are they now? Check their career progress on Dr. Carpenter's [ CV] Note: the Carpenter lab became the Carpenter-Singh lab in 2021. Alum Years Role Adam Fraser 2008–2011 Lead software developer for the CellProfiler Analyst project Adam Papallo 2005–2007 Bioinformatics Analyst Adeniyi Adeboye...

CellProfiler 3.0 release: faster, better, and 3D

Blog: October 16, 2017

Anne Carpenter We are thrilled to announce that CellProfiler 3.0 is now released! Download it here. Eighteen months in the making, this is the first version of CellProfiler that can identify objects in 3D images volumetrically – the result of a collaboration with the Allen Institute for Cell Science...

Quantifying microscopy images: top 10 tips for image acquisition

Blog: June 15, 2017

Anne Carpenter Not every image you capture on your microscope is suited for quantification, no matter how nice they may look. Even though you might not notice any problems by eye, the tips outlined here for acquiring and storing images can improve the quality of data derived from digital image...