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Human MCF7 cells – compound-profiling experiment

Accession number BBBC021 · Version 1 Example Images Actin disrupter Aurora kinase inhbitor Eg5 inhibitor Tubulin destabilizer Tubulin stabilizer Description of the biological application Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each...

Human U2OS cells – Gene overexpression profiling experiment

Accession number BBBC037 · Version 1 Example images Description of the biological application The image set can be used to functionally annotate the genes through comparing their overexpression morphological profiles. Images Images from five fluorescent channels were captured at 20x magnification on...

C. elegans metabolism assay

Accession number BBBC011 · Version 1 Example images Negative control: wild type Positive control: fat Biological application Images are controls from an RNA interference screen for genes affecting fat mass in the nematode C.elegans. The animals are stained with oil red O, a non-flourescentdye which...

Human U2OS cells cytoplasm–nucleus translocation

Accession number BBBC013 · Version 1 Example images Positive Negative Biological application This 96-well plate has images of cytoplasm to nucleus translocation of the Forkhead (FKHR-EGFP) fusion protein in stably transfected human osteosarcoma cells, U2OS. In proliferating cells, FKHR is localized...

Human U2OS cells transfluor

Accession number BBBC015 · Version 1 Example images Positive Negative Biological application The images are of a human osteosarcoma cell line (U2OS) co-expressing beta2 (b2AR) adrenergic receptor and arrestin-GFP protein molecules. The receptor was modified-type that generates "vesicle-type" spots...

Human U2OS cells transfluor

Accession number BBBC016 · Version 1 Example images Positive Negative Description of the biological application This image set is of a Transfluor assay where an orphan GPCR is stably integrated into the b-arrestin GFP expressing U2OS cell line. After one hour incubation with a compound the cells...

Mouse trophoblast stem cells

Accession number BBBC033 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered. Mouse trophoblast stem cells image contains a clustered monolayer of nuclei that can be difficult to segment. This...

Simulated HL60 cells (from the Cell Tracking Challenge)

Accession number BBBC035 · Version 1 Example images Description of the biological application These are synthetic images from the Cell Tracking Challenge. The images depict simulated nuclei of HL60 cells stained with Hoescht (training datasets). These synthetic images of HL60 cells provide an...

Human MCF7 and A549 cells cytoplasm–nucleus translocation

Accession number BBBC014 · Version 1 Example images Positive Negative Biological application This 96-well plate has images of cytoplasm to nucleus translocation of the transcription factor NFκB in MCF7 (human breast adenocarcinoma cell line) and A549 (human alveolar basal epithelial) cells in...

P. vivax (malaria) infected human blood smears

Accession number BBBC041 · Version 1 Example images Description of the biological application Malaria is a disease caused by Plasmodium parasites that remains a major threat in global health, affecting 200 million people and causing 400,000 deaths a year. The main species of malaria that affect...