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Accession number BBBC007 · Version 1 Example images DNA Actin Outlined nuclei Outlined cells Biological application Drosophila melanogaster Kc167 cells were stained for DNA (to label nuclei) and actin (a cytoskeletal protein, to show the cell body). Automatic cytometry requires that cells be...

Accession number BBBC039 · Version 1 Example images Description of the biological application This image set is part of a high-throughput chemical screen on U2OS cells, with examples of 200 bioactive compounds. The effect of the treatments was originally imaged using the Cell Painting assay...

Accession number BBBC012 · Version 1 Example images Positive: pmk-1(km25) Negative: wild type Biological application This selection of images show transgenic C. elegans expressing the promoter of gene clec-60 fused to GFP. The clec-60 gene is up-regulated during S. aureus infection [Irazoqui et al...

Workflow Turnaround The Broad CMG provides exome and/or whole­ genome sequencing at no cost to the collaborator for affected cases and relatives. This centralized service offers high­ quality sequencing and analysis, incorporating discovery of all classes of variation (including copy number variants...

We welcome contributions of the following: Image sets with descriptions and ground truth High-throughput image sets Published benchmarking results of algorithms applied to the BBBC image sets Corrections to existing descriptions or ground truth Feedback on the benchmarking methodology or other...

CellProfiler Analyst (CPA) provides tools for classifying biological images and exploring and visualizing multidimensional data (particularly from high-throughput experiments) that has been extracted from companion image analysis software CellProfiler. CellProfiler Analyst’s primary tools: Image...

Accession number BBBC008 · Version 1 Example images Biological application These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. A set of about 43,000 such images was used by Moffat et al. (Cell, 2006) to...

Accession number BBBC020 · Version 1 Note: BBBC has received a complaint that "BBB020_v1_outlines_nuclei" appears incomplete and we have been unable to obtain the missing images from the original contributor. Example Images Merged CD11b/APC DAPI Biological application These images depict bone-marrow...

Accession number BBBC029 · Version 1 Example images DIC images Ground truth Images The dataset includes 218 simulated Differential Interference Contrast (DIC) images of 20 different binary and grayscale objects with various rotations. The images are available in noise-free version as well as with 20...

Accession number BBBC032 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered not only in XY plane but also in XZ and YZ planes. Manually annotated ground truth provides a reference for image...