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Pearl V. Ryder One thing I’ve found very helpful in my journey as an image analyst is learning from others by observing their thought process when they tackle a new problem. In this series of five blog posts, my goal is to share how I approached an image analysis project that I recently undertook as...

David R. Stirling Hi everyone, Today we’re releasing the RunCellpose plugin for CellProfiler 4! This plugin is designed to allow you to use the popular Cellpose segmentation algorithm to generate object sets within a CellProfiler pipeline. Cellpose uses a neural network followed by post-processing...

Pearl V. Ryder In the first post of this series, I gave an overview of this project and explained how I imported the data into CellProfiler. If you’d like to follow along in CellProfiler, the pipeline and images for this project are available here. Now that the images have been imported, I could...

Pearl V. Ryder The Robust Background algorithm is a powerful algorithm for automatically setting thresholds to segment objects of interest when your image contains mostly background. However, since it contains the largest number of tunable parameters of any thresholding algorithm in CellProfiler, it...