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Accession number BBBC013 · Version 1 Example images Positive Negative Biological application This 96-well plate has images of cytoplasm to nucleus translocation of the Forkhead (FKHR-EGFP) fusion protein in stably transfected human osteosarcoma cells, U2OS. In proliferating cells, FKHR is localized...

Accession number BBBC015 · Version 1 Example images Positive Negative Biological application The images are of a human osteosarcoma cell line (U2OS) co-expressing beta2 (b2AR) adrenergic receptor and arrestin-GFP protein molecules. The receptor was modified-type that generates "vesicle-type" spots...

Accession number BBBC016 · Version 1 Example images Positive Negative Description of the biological application This image set is of a Transfluor assay where an orphan GPCR is stably integrated into the b-arrestin GFP expressing U2OS cell line. After one hour incubation with a compound the cells...

We welcome contributions of the following: Image sets with descriptions and ground truth High-throughput image sets Published benchmarking results of algorithms applied to the BBBC image sets Corrections to existing descriptions or ground truth Feedback on the benchmarking methodology or other...

Accession number BBBC008 · Version 1 Example images Biological application These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. A set of about 43,000 such images was used by Moffat et al. (Cell, 2006) to...

Accession number BBBC020 · Version 1 Note: BBBC has received a complaint that "BBB020_v1_outlines_nuclei" appears incomplete and we have been unable to obtain the missing images from the original contributor. Example Images Merged CD11b/APC DAPI Biological application These images depict bone-marrow...

Accession number BBBC029 · Version 1 Example images DIC images Ground truth Images The dataset includes 218 simulated Differential Interference Contrast (DIC) images of 20 different binary and grayscale objects with various rotations. The images are available in noise-free version as well as with 20...

Accession number BBBC032 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered not only in XY plane but also in XZ and YZ planes. Manually annotated ground truth provides a reference for image...

Accession number BBBC034 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered and have different intensity. This image set contains a clustered monolayer of nuclei that can be difficult to segment...

Accession number BBBC010· Version 1 Example images Positive: mostly alive Negative: mostly dead Biological application This selection of images are controls selected from a screen to find novel anti-infectives using the roundworm C.elegans . The animals were exposed to the pathogen Enterococcus...