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Accession number BBBC033 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered. Mouse trophoblast stem cells image contains a clustered monolayer of nuclei that can be difficult to segment. This...

Accession number BBBC035 · Version 1 Example images Description of the biological application These are synthetic images from the Cell Tracking Challenge. The images depict simulated nuclei of HL60 cells stained with Hoescht (training datasets). These synthetic images of HL60 cells provide an...

Accession number BBBC014 · Version 1 Example images Positive Negative Biological application This 96-well plate has images of cytoplasm to nucleus translocation of the transcription factor NFκB in MCF7 (human breast adenocarcinoma cell line) and A549 (human alveolar basal epithelial) cells in...

Accession number BBBC041 · Version 1 Example images Description of the biological application Malaria is a disease caused by Plasmodium parasites that remains a major threat in global health, affecting 200 million people and causing 400,000 deaths a year. The main species of malaria that affect...

Accession number BBBC022 · Version 1 Example Images Hoechst 33342 staining con A staining SYTO 14 staining WGA + phalloidin staining MitoTracker Deep Red staining Description of the biological application Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular...

Accession number BBBC046 · Version 1 Example images The synthetic time-lapse image data of a CRMP-2-phospho-defective cell for the given fluorescence level facto(F) and anisotropy ratio (AR). The synthetic image data of a CRMP-2-phospho-defective cell for varying anisotropy ratios (ARs). The...

We welcome contributions of the following: Image sets with descriptions and ground truth High-throughput image sets Published benchmarking results of algorithms applied to the BBBC image sets Corrections to existing descriptions or ground truth Feedback on the benchmarking methodology or other...

Accession number BBBC008 · Version 1 Example images Biological application These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. A set of about 43,000 such images was used by Moffat et al. (Cell, 2006) to...

Accession number BBBC020 · Version 1 Note: BBBC has received a complaint that "BBB020_v1_outlines_nuclei" appears incomplete and we have been unable to obtain the missing images from the original contributor. Example Images Merged CD11b/APC DAPI Biological application These images depict bone-marrow...