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Imaging flow cytometry (IFC) combines the high-throughput capabilities of conventional flow cytometry with single-cell imaging. CellProfiler can be used to analyze the resulting images from imaging flow cytometry, whether brightfield, darkfield, or fluorescence. We here provide an open-source IFC...

A component of CellProfiler cell image analysis software The WormToolbox is a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and...

CellProfiler enables reproducible research because a saved pipeline includes all the modules and settings. Here, we provide links to pipelines used in some published studies, as well as the version of CellProfiler used to create them. You can instead download the most current version of CellProfiler...

OLD CellProfiler example images and pipelines (Prior to 4.0) These examples are outdated, please see our current Examples page for the updated versions. Basic Pipelines Human cells Fruit fly cells Tumors Comet assay Specialized Pipelines Cell/particle counting and scoring the percentage of stained...

Accession number BBBC003 · Version 1 Example images . DIC Image Foreground Biological application Fluorescence microscopy cannot be used to image human embryos to determine embryo viability for in vitro fertilization because the introduction of exogenous fluorescent dyes is considered a toxic...

Accession number BBBC010 · Version 1 Example images Positive: mostly alive Negative: mostly dead Biological application This selection of images are controls selected from a screen to find novel anti-infectives using the roundworm C.elegans . The animals were exposed to the pathogen Enterococcus...

Accession number BBBC018 · Version 1 Example Images ​ ​ ​ ​ ​ Actin blebs Actin dots Anaphase/Telephase Angular Cell Edges Cresent Nuclei ​ ​ ​ ​ ​ Long Projections Metaphase Motile Peas in a Pod Peripheral Actin ​ ​ ​ ​ Large Spread Phospho-histone H3 dots Prometaphase Prophase Biological...

Accession number BBBC030 · Version 1 Example images DIC image Ground truth Images The image set consists of 60 Differential Interference Contrast (DIC) images of Chinese Hamster Ovary (CHO) cells. The images are taken on an Olympus Cell-R microscope with a 20x lens at the time when the cell...

Accession number BBBC011 · Version 1 Example images Negative control: wild type Positive control: fat Biological application Images are controls from an RNA interference screen for genes affecting fat mass in the nematode C.elegans. The animals are stained with oil red O, a non-flourescentdye which...

Accession number BBBC013 · Version 1 Example images Positive Negative Biological application This 96-well plate has images of cytoplasm to nucleus translocation of the Forkhead (FKHR-EGFP) fusion protein in stably transfected human osteosarcoma cells, U2OS. In proliferating cells, FKHR is localized...