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The CellProfiler project team is based in the Cimini Lab at the Broad Institute of MIT and Harvard. The project was started in 2003 by Anne E. Carpenter and Thouis (Ray) Jones in the Sabatini Laboratory (Whitehead Institute for Biomedical Research) and Golland laboratory (MIT’s CSAIL) and is...

Our CellProfiler software has yielded discoveries in several translational projects, some of which have had a direct impact on the treatment of disease. For example, CellProfiler has been used to identify several small molecules that are effective in treating particular diseases in mouse models. In...

Imaging flow cytometry (IFC) combines the high-throughput capabilities of conventional flow cytometry with single-cell imaging. CellProfiler can be used to analyze the resulting images from imaging flow cytometry, whether brightfield, darkfield, or fluorescence. We here provide an open-source IFC...

A component of CellProfiler cell image analysis software The WormToolbox is a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and...

CellProfiler enables reproducible research because a saved pipeline includes all the modules and settings. Here, we provide links to pipelines used in some published studies, as well as the version of CellProfiler used to create them. You can instead download the most current version of CellProfiler...

OLD CellProfiler example images and pipelines (Prior to 4.0) These examples are outdated, please see our current Examples page for the updated versions. Basic Pipelines Human cells Fruit fly cells Tumors Comet assay Specialized Pipelines Cell/particle counting and scoring the percentage of stained...

CellProfiler Video Tutorials Visit our YouTube playlist for video tutorials on CellProfiler, CellProfiler Analyst, segmentation strategies, how to construct pipelines, and much more. CellProfiler Written Tutorials Technical descriptions of CellProfiler and CellProfiler Analyst software can be found...

Note: CellProfiler versions 2.2 and 3.0 require installation of a Java 8 JDK CellProfiler 4 4.2.7 macOS (10.15+) Windows (64-bit) 4.2.6 macOS (10.15+) Windows (64-bit) 4.2.5 macOS (10.15+) Windows (64-bit) 4.2.4 macOS (10.15+) Windows (64-bit) 4.2.1 macOS (10.14+) Windows (64-bit) 4.1.3 macOS (10.14...