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Customizing a Model for Fiber Segmentation, Part 4: Analyzing the Results

Melissa Gillis In the final section of this blogpost series, I will discuss some of the benefits and drawbacks of this model and discuss some of the data I acquired from the images. This model was able to successfully segment the images that were not able to be accurately segmented by Pearl’s...

Community’s Input: 2020 BioImage Analysis Survey

Nasim Jamali As creators of open source tools, we spend a lot of time thinking about user needs - what kinds of images people are working with these days, what tools are working well, where the pain points are, and how easy it is to learn how to do any of this in the first place. While things like...

Thinking like an image analyst, Part IV: Detecting fibers as objects

Pearl V. Ryder In the previous post in this “Thinking like an image analyst” series, I explained how I enhanced fibers to increase their brightness and applied a background subtraction to decrease the intensity of the background. In combination with masking out very bright debris pixels ( Part II of...

Shantanu Singh

I lead the image-based profiling team. We develop machine learning and statistical methods to create fingerprints of genes, chemicals, and diseases from microscopy images of cells.

How to Normalize Cell Painting Data

Rebecca Senft Workflow update June 2024 update Our recommended normalization workflow is evolving. Please see https://github.com/broadinstitute/jump-profiling-recipe/ for our latest developments on the normalization strategy in specific and aprofiling workflow in general. How to Normalize Cell...

Runxi Shen

I focus on developing advanced deep-learning methods to create robust cell representations from cell painting assays. My research aims to understand how various genetic variants alter cellular states and explore potential treatments for diseases associated with these variants.

Press from the Imaging Platform (Carpenter-Singh and Cimini labs)

Impact of CellProfiler on human health Our CellProfiler software has yielded discoveries in several translational projects, some of which may ultimately have a direct impact on the treatment of disease [ read more here]. Public videos of talks and PDF slides Links to PDFs of slides from recent talks...

Papers

For our latest work, check out our preprints on bioRxiv! ( Carpenter) ( Singh) Google Scholar pages (with citation metrics): ( Carpenter) ( Singh) 204. Seal S, Mahale M, García-Ortegón M, Joshi CK, Hosseini-Gerami L, Beatson A, Greenig M, Shekhar M, Patra A, Weis C, Mehrjou A, Badré A, Paisley B...

Announcing CellProfiler 3.1.8

Beth Cimini Happy holidays to everyone- we here at the CellProfiler team got you a little end-of-year treat in the form of CellProfiler 3.1.8. This is primarily a bugfix release, getting rid of some bugs in MeasureObjectIntensity, MeasureColocalization, ExportToSpreadsheet...