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Synthetic cells

Accession number BBBC005 · Version 1 Example images In focus Out of focus True foreground Biological application Since robust foreground/background separation and segmentation of cellular objects (i.e.,identification of which pixels below to which objects) strongly depends on image quality, focus...

Drosophila Kc167 cells

Accession number BBBC007 · Version 1 Example images DNA Actin Outlined nuclei Outlined cells Biological application Drosophila melanogaster Kc167 cells were stained for DNA (to label nuclei) and actin (a cytoskeletal protein, to show the cell body). Automatic cytometry requires that cells be...

Nuclei of U2OS cells in a chemical screen

Accession number BBBC039 · Version 1 Example images Description of the biological application This image set is part of a high-throughput chemical screen on U2OS cells, with examples of 200 bioactive compounds. The effect of the treatments was originally imaged using the Cell Painting assay...

C. elegans infection marker

Accession number BBBC012 · Version 1 Example images Positive: pmk-1(km25) Negative: wild type Biological application This selection of images show transgenic C. elegans expressing the promoter of gene clec-60 fused to GFP. The clec-60 gene is up-regulated during S. aureus infection [Irazoqui et al...

Workflow

Workflow Turnaround The Broad CMG provides exome and/or whole­ genome sequencing at no cost to the collaborator for affected cases and relatives. This centralized service offers high­ quality sequencing and analysis, incorporating discovery of all classes of variation (including copy number variants...