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The CellProfiler project team is based in the Cimini Lab at the Broad Institute of MIT and Harvard. The project was started in 2003 by Anne E. Carpenter and Thouis (Ray) Jones in the Sabatini Laboratory (Whitehead Institute for Biomedical Research) and Golland laboratory (MIT’s CSAIL) and is...

Human HT29 colon-cancer cells

Accession number BBBC001 · Version 1 Example image Biological application These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. For instance, Moffat et al. ( Cell, 2006) screened a set of about 43,000 such...

Synthetic cells

Accession number BBBC004· Version 1 Example images Image Foreground Biological application One of the principal challenges in counting or segmenting nuclei is dealing with clustered nuclei. To help assess algorithms' performance in this regard, this synthetic image set consists of five subsets with...

Human red blood cells

Accession number BBBC009 · Version 1 Example images DIC image Ground truth Images This image set consists of five differential interference contrast (DIC) images of red bood cells. BBBC009_v1_images.zip (1.2 MB) Ground truth BBBC009_v1_outlines.zip (86 kB) There are two options when using this data...

3D Colon Tissue (synthetic data)

Accession number BBBC027 · Version 1 Example images 3D image 3D foreground Biological application One of the principal challenges in counting or segmenting nuclei is dealing with clustered nuclei. To help assess algorithms' performance in this regard, this synthetic image set is provided in two...

Polymerized structures

Accession number BBBC028 · Version 1 Example images DIC image Ground truth Images This dataset includes 60 Differential Interference Contrast (DIC) images of 6 different shapes, such as triangle, circle and multi-height objects. The shapes were made by two-photon polymerization technique. The images...

Kaggle 2018 Data Science Bowl

Accession number BBBC038 · Version 1 Example images Description of the biological application This image data set contains a large number of segmented nuclei images and was created for the Kaggle 2018 Data Science Bowl sponsored by Booz Allen Hamilton with cash prizes. The image set was a testing...

P. vivax (malaria) infected human blood smears

Accession number BBBC041· Version 1 Example images Description of the biological application Malaria is a disease caused by Plasmodium parasites that remains a major threat in global health, affecting 200 million people and causing 400,000 deaths a year. The main species of malaria that affect...

Rat astrocyte cells

Accession number BBBC042 · Version 1 Example images Description of the biological application The morphology of astrocytes is highly heterogeneous and counting them is currently a laborious task. Development of fully automated counting methods for astrocytes from immunohistological images without...