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Induced pluripotent human stem cells

Accession number BBBC034 · Version 1 Example images Description of the biological application Segmenting nuclei in 3D images can be challenging especially when nuclei are clustered and have different intensity. This image set contains a clustered monolayer of nuclei that can be difficult to segment...

Simulated 24-well plate with synthetic cells

Accession number BBBC031 · Version 1 Example images Description of the biological application We generated a synthetic HCS dataset simulating drugs perturbing the cell shape and the expressions of proteins (here represented by different colours). The dataset is useful for testing classification and...

Human U2OS cells – Small molecule Cell Painting experiment

Accession number BBBC040 · Version 1 Example images Biological application An ideal small-molecule screening library would be enriched in non-redundant bioactive compounds that maximize the performance diversity of the library. The construction of such libraries would be aided by a rapid feedback...

Community Enagagment

COBA’s mission is to catalyze the entire bioimage analysis software community. What have we accomplished thus far? Scientific Community Image Forum We created and sponsor the Scientific Community Image Forum. This forum has brought together dozens of open source image analysis platforms to one...

Nuclei of mouse embryonic cells

Accession number BBBC050 · Version 2 Example images Biological application Cell dynamics during the early mouse embryogenesis change spatiotemporally. For understanding the mechanism of this developmental process, imaging cell dynamics by live-cell imaging of fluorescently labeled nuclei and...

Research

KIDNEY DISEASE Autosomal dominant MUC1 kidney disease (MKD, also known as ADTKD-MUC1, and formerly called MCKD1) is a rare hereditary renal disease that ultimately requires dialysis or kidney transplantation for patients. For decades, scientists had known the genetic “neighborhood” of the mutation...

Software and code

CellProfiler and CellProfiler Analyst Our lab previously launched and led the CellProfiler and CellProfiler Analyst projects, beloved by biologists around the world for bioimage analysis and cited over 15,000 times. They are now developed and maintained by the Cimini lab (since 2021). DeepProfiler ​...

Promo Images

Biographical images are available here with credits noted in each folder name. Permission to use those or any images on this page must be requested via imagingadmin@broadinstitute.org.

Getting Started

CellProfiler is free, open-source software for quantitative analysis of biological images. No prior experience in programming or computer vision is required – this page is intended to help you get up and running. The quickest way to get up and running with CellProfiler is to: Download CellProfiler...