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Email imagingadmin@broadinstitute.org Mailing address Broad Institute of MIT and Harvard415 Main StreetCambridge, MA 02142Our offices are on the 5th floor of 415 Main St [ Google Maps link]. Look for the entrance of our building to the right of the Broad Institute sign.

NSF CAREER Award Project (March 2012–February 2017) [ Project abstract at nsf.gov] [Project Outcomes Report (search here, using Fed Award ID 1148823 ( PDF))] Non-technical description of the problem: Biologists want to test genetic and chemical treatments in environments that more closely mimic the...

Current Openings: Thank you for your interest. Please keep an eye on this page for openings. We welcome inquiries from students and experienced scientists at all levels. Please send your inquiries to TRGapplications@broadinstitute.org, and please be sure to include what position you are interested...

Version Manual CellProfiler 4.2.8 [ HTML] 4.2.7 [ HTML] 4.2.6 [ HTML] 4.2.5 [ HTML] 4.2.4 [ HTML] 4.2.1 [ HTML] 4.2.0 [ HTML ] 4.1.3 [ HTML ] 4.0.7 [ HTML] 3.1.9 [ HTML ] 3.1.8 [ HTML ] 3.1.5 [ HTML ] 3.0 [ HTML ] 2.1 and 2.2 [ HTML ] 2.0 [ PDF ] 1.0 [ PDF ]

Broad CMG publications To view all Broad CMG publications in PubMed, please click here. Publication Policy In general, it is anticipated that collaborators will retain first and senior authorship for straightforward discoveries made on their samples (either discovered by the core Broad CMG staff or...

Where are they now? Check their career progress on Dr. Carpenter's [ CV] Note: the Carpenter lab became the Carpenter-Singh lab in 2021. Alum Years Role Adam Fraser 2008–2011 Lead software developer for the CellProfiler Analyst project Adam Papallo 2005–2007 Bioinformatics Analyst Adeniyi Adeboye...

Previous Examples Previous examples (prior to 4.0) can be found on the previous examples page, but we discourage their use because they are now outdated.

To reasonably compare, or benchmark, algorithms for image analysis, researchers must agree on a common reference point—the image set's "correct" answer—and see how closely each algorithm matches it. We adopt the common term ground truth for this reference point even though it may be questionable in...

Accession number BBBC002 · Version 1 Example image Biological application Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and...

Accession number BBBC006 · Version 1 Example images Ground truth: in focus Out of focus Ground truth: foreground Biological application Since robust foreground/background separation and segmentation of cellular objects (i.e., identification of which pixels below to which objects) strongly depends on...