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WormToolbox

A component of CellProfiler cell image analysis software The WormToolbox is a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and...

Previous Examples

OLD CellProfiler example images and pipelines (Prior to 4.0) These examples are outdated, please see our current Examples page for the updated versions. Basic Pipelines Human cells Fruit fly cells Tumors Comet assay Specialized Pipelines Cell/particle counting and scoring the percentage of stained...

Mouse Embryos

Accession number BBBC003 · Version 1 Example images . DIC Image Foreground Biological application Fluorescence microscopy cannot be used to image human embryos to determine embryo viability for in vitro fertilization because the introduction of exogenous fluorescent dyes is considered a toxic...

Chinese Hamster Ovary Cells

Accession number BBBC030 · Version 1 Example images DIC image Ground truth Images The image set consists of 60 Differential Interference Contrast (DIC) images of Chinese Hamster Ovary (CHO) cells. The images are taken on an Olympus Cell-R microscope with a 20x lens at the time when the cell...

C. elegans metabolism assay

Accession number BBBC011 · Version 1 Example images Negative control: wild type Positive control: fat Biological application Images are controls from an RNA interference screen for genes affecting fat mass in the nematode C.elegans. The animals are stained with oil red O, a non-flourescentdye which...

Human U2OS cells cytoplasm–nucleus translocation

Accession number BBBC013 · Version 1 Example images Positive Negative Biological application This 96-well plate has images of cytoplasm to nucleus translocation of the Forkhead (FKHR-EGFP) fusion protein in stably transfected human osteosarcoma cells, U2OS. In proliferating cells, FKHR is localized...

Human U2OS cells transfluor

Accession number BBBC015 · Version 1 Example images Positive Negative Biological application The images are of a human osteosarcoma cell line (U2OS) co-expressing beta2 (b2AR) adrenergic receptor and arrestin-GFP protein molecules. The receptor was modified-type that generates "vesicle-type" spots...

Human U2OS cells transfluor

Accession number BBBC016 · Version 1 Example images Positive Negative Description of the biological application This image set is of a Transfluor assay where an orphan GPCR is stably integrated into the b-arrestin GFP expressing U2OS cell line. After one hour incubation with a compound the cells...

Tutorials

CellProfiler Video Tutorials Visit our YouTube playlist for video tutorials on CellProfiler, CellProfiler Analyst, segmentation strategies, how to construct pipelines, and much more. CellProfiler Written Tutorials Technical descriptions of CellProfiler and CellProfiler Analyst software can be found...