The Optical Profiling Platform at the Broad Institute develops advanced imaging methods for high-throughput generation of integrated, multimodal portraits of biological systems with single-cell precision. We focus on highly multiplexed in situ proteomics and transcriptomics; optical barcoding technologies for in situ screening; and assays for high dimensional functional profiling, especially all optical electrophysiology. We use these tools to understand the gene-phenotype interactions misregulated in psychiatric disease, especially autism spectrum disorder and Schizophrenia; and to elucidate the cell-to-cell interactions underlying tissue structure in cancer and immunological disease.
A major activity in our lab is the improvement, comparison, and dissemination of emerging imaging techniques to members of the scientific community. This facilitates large scale collaborations with the Broad Intitute's Klarman Cell Observatory, Stanley Center of Psychiatric Research, and Gene Regulation Observatory, as well as with hospitals and institutions in the Boston area. Nationally, this work underlies our participation in the Brain Initiative Cell Census Network, the Human Tumor Atlas Network, and Human Cell Atlas.
Our group is constantly growing as a mix of academic trainees and professional scientists, with expertise spanning experimental biology, engineering, and computer science.