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Preguntas frecuentes

Explore esta página para hallar respuestas a preguntas frecuentes. También le recomendamos que lea las demás páginas de este sitio web. Si no encuentra respuesta a su pregunta, escríbanos por correo electrónico a raregenomes@broadinstitute.org o llámenos por teléfono al 617-714-7395 (Línea gratuita...

Funding

Since 2003, grants have been provided by... ​ ​ ​​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​

Human MCF7 cells – compound-profiling experiment

Accession number BBBC021 · Version 1 Example Images Actin disrupter Aurora kinase inhbitor Eg5 inhibitor Tubulin destabilizer Tubulin stabilizer Description of the biological application Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each...

Human U2OS cells – Gene overexpression profiling experiment

Accession number BBBC037 · Version 1 Example images Description of the biological application The image set can be used to functionally annotate the genes through comparing their overexpression morphological profiles. Images Images from five fluorescent channels were captured at 20x magnification on...

Imaging flow cytometry analysis using CellProfiler

Imaging flow cytometry (IFC) combines the high-throughput capabilities of conventional flow cytometry with single-cell imaging. CellProfiler can be used to analyze the resulting images from imaging flow cytometry, whether brightfield, darkfield, or fluorescence. We here provide an open-source IFC...

WormToolbox

A component of CellProfiler cell image analysis software The WormToolbox is a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and...

Previous Examples

OLD CellProfiler example images and pipelines (Prior to 4.0) These examples are outdated, please see our current Examples page for the updated versions. Basic Pipelines Human cells Fruit fly cells Tumors Comet assay Specialized Pipelines Cell/particle counting and scoring the percentage of stained...

Mouse Embryos

Accession number BBBC003 · Version 1 Example images . DIC Image Foreground Biological application Fluorescence microscopy cannot be used to image human embryos to determine embryo viability for in vitro fertilization because the introduction of exogenous fluorescent dyes is considered a toxic...

C. elegans live/dead assay

Accession number BBBC010 · Version 1 Example images Positive: mostly alive Negative: mostly dead Biological application This selection of images are controls selected from a screen to find novel anti-infectives using the roundworm C.elegans . The animals were exposed to the pathogen Enterococcus...